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Larissa Marila de Souza1,2, Natalia Mayumi Inada2, Sebastião Pratavieira2, Juliano José Corbi3, Cristina Kurachi2 and Vanderlei Salvador Bagnato2


1. Federal University of São Carlos, PPG Biotec, São Carlos 13565-905, São Paulo, Brazil
2. São Carlos Institute of Physics, University of São Paulo, São Carlos 13560-970, São Paulo, Brazil
3. São Carlos School of Engineering, University of São Paulo, São Carlos 13560-970, São Paulo, Brazil


Dengue vector is responsible for millions of deaths every year and has caused disastrous impacts on health systems. The continuous use of chemical insecticides, such as carbamates, pyrethroids and organophosphates generates resistant populations of the mosquito, therefore, new control methods must be investigated. The joint action of the population and guidelines for preventing the reproduction of the mosquito associated with the use of photoactivatable insecticides can be the alternative for the control of epidemiological outbreaks in affected regions. In this study, the photo-larvicidal activity of Photogem®(PG), a derivative of hematoporphyrin, was investigated against 2nd–early 3rd instar of Aedes aegypti larvae (Diptera: Culicidae) under different lighting conditions (artificial lighting system and sunlight). The dynamics of PG accumulation was characterized by CLSM (confocal laser scanning microscopy) and total time PG eliminationin solution was investigated by ultraviolet-visible spectrophotometry. The maximum photo-activity of PG was observed in 0.5 h under sunlight exposure which achieved 100% larval mortality. Fluorescence images showed a uniform distribution of PG along the digestive tract. PG remained stable in the sunlight for 48 h and in an artificial lighting system for longer periods, therefore, it can be used for the control of Aedes aegypti larvae as a new alternative to chemical insecticides. The method is considered environmentally friendly due to its rapid degradation in the presence of light. Further studies are required, so that the potential of the technique can be explored in real breeding places.


Photoactivatable larvicide, PG, hematoporphyrin derivative, Aedes aegypti, natural lighting, artificial lighting. 

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